Arch Pathol Lab Med. 2003 Feb;127(2):200-4.
Comment in:

·   Arch Pathol Lab Med. 2003 Feb;127(2):143-5.

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·                           Comparative analysis of conventional Papanicolaou tests and a fluid-based thin-layer method.

Limaye A, Connor AJ, Huang X, Luff R.
Department of Anatomic Pathology, Quest Diagnostics Incorporated, Teterboro, NJ 07608-1070, USA. limayea@questdiagnostics.com

CONTEXT: A fluid-based, direct-to-vial method of thin-layer gynecologic cytology (ThinPrep Pap Test) is reported to be more effective than the conventional Papanicolaou test in the detection of squamous intraepithelial lesions. OBJECTIVE: This retrospective analysis evaluated the validity of the findings on the thin-layer method using case material at a large independent laboratory and represented a comparison of performance of both methods over an identical period. METHODS: Data for conventional and ThinPrep tests were compared for 2 periods. Period 1 included 1,421,080 conventional and 56,835 ThinPrep specimens, and period 2 included 564,270 conventional and 109,784 ThinPrep specimens. Squamous intraepithelial lesions were used to determine detection of disease. These 2 sets of data were also analyzed to eliminate effects of any selection bias toward ThinPrep for high-risk patients. RESULTS: Use of ThinPrep showed a greater than 100% increase in the detection rate of squamous intraepithelial lesions (1.3%-3.4% in period 1 and 1.3%-2.9% in period 2), which was statistically significant after correcting for selection bias. We also found a significant decrease in the false-negative proportion (57% in period 1 and 35% in period 2). There was a marked improvement (233%) in the detection of high-grade squamous intraepithelial lesions in high-risk cases and a decrease in the atypical squamous cells of undetermined significance to squamous intraepithelial lesion ratio from 3.1 to 1.5 in period 2. CONCLUSION: ThinPrep is better than the conventional Papanicolaou test in detecting squamous intraepithelial lesions and is a superior screening test in detection of precancerous changes of the cervix.

传统涂片和膜式液基薄层方法的比较分析

摘要：评价直接进行液基薄层妇科细胞学Thinprep测试在检测鳞状上皮内病变方面比传统巴氏涂片方法的更加有效性。

 

目的：此回顾性研究是评价液基薄层细胞学和传统涂片两种方法所制片的有效性。此研究是通过典型病例在确定的一个时期和各自大型独立实验室进行。

方法：来自于两个时期的传统和新柏氏两种方法的数据进行比较。第一个时间段传统涂片共有1，421，080例参与测试，新柏氏有56835例；第二个时间段传统涂片共有564，270例参与测试，新柏氏有109，784例标本。鳞状上皮内瘤变即为需要检测到的病变分析这两套数据也用来消除认为新柏氏TCT对高危病人检测有效性的人为偏见。

结果：用TCT测试可对检测鳞状上皮内瘤变的检出率提高100%以上（第一时间段研究结果传统对新柏氏：1.3%VS.3.4%,第二时间段为1.3%~2.9%）,这些统计数据对于消除选择检测方法的偏见性是非常有意义的。我们也发现用TCT后假阴性的比例极大的降低了（第一时间段降低了57%，第二时间段降低了35%）。对于第二时间段新柏氏对高度鳞状上皮内瘤变的检出率由一个显著的提高为233%，ASCUS/SIL有一个很大的降低，即从3.1降到了1.5.

 

结论：在检测鳞状上皮内病变时新柏氏技术要好于传统涂片方法，新柏氏在宫颈癌前病变筛查中是一个更好的方法。